It is essential to know that storage varies based on the kind of peptide in question. Some need dry storage, while others necessitate liquid storage. Peptide storage is an essential skill for every researcher, but it’s imperative when working with many peptide forms at once. The following guidelines should help you figure out how to store research peptides. Peptides must be handled and stored in circumstances that prevent degradation over many months or years.
Whether peptides are lyophilized or in solution, we’ll show you how to store peptides to get the most out of them in this article.
Introduction To Peptides
The polymer of amino acid is a peptide. Small amounts of proteins that have little or no action are expected. As peptides in cytokines and hormones, they are also signaling molecules that interact with specific receptors.
Solid Phase Peptide Synthesis (SPPS), invented by chemist Bruce Merrifield in 1963, allows you to synthesize peptides. This method would be used to produce peptides in any business in the future. Each peptide chain is attached to a tiny polystyrene bead using this technique. This allows you to store the peptides in a container and perform chemistry on them. The resin with the peptide attached can be drained while a filter in the vessel retains the solution.
Dry peptide storage
There are two ways to store peptides when they are dry. Leaving the peptide at room temperature as a short-term storage solution is the first option available. However, this can be done if the peptide is stable at room temperature.
If the peptide isn’t stable at room temperature, the second alternative is to chill it to stay stable for a brief period. Keeping the peptide around 4 degrees Fahrenheit can suffice for a few months, but you’ll need to keep it at a lower temperature if you’re storing it for a lengthy time.
Solution-based storage of peptides
Long-term storage of liquid peptides is never an easy feat. In order to store peptides for an extended period, it is best to freeze them at temperatures below 200C and keep them refrigerated for the time being. For long-term stability and accurate findings, the peptide must be protected against degradation and degeneration under these circumstances.
For this reason, it is suggested that researchers only buy the peptide amounts they will utilize. A peptide’s integrity may be affected by repeated freezing and thawing when stored in refrigeration for an extended period.
Keeping Peptides Lyophilized
The general guideline is that lyophilized peptides may be kept at ambient temperature or refrigerated at +4C for many weeks if they are to be used within this period. Keeping them away from bright light sources is critical in this instance.
To minimize bacterial deterioration, oxidation, and the creation of secondary structures, peptides stored for more than four weeks should be frozen at -20°C or -80°C.
Consideration should also be given to the sequence that makes up each peptide, one of the most critical variables in determining their stability.
- Cys, Met, and Trp-containing peptides must be kept under anaerobic conditions because of their sensitivity to oxidation.
- Peptides containing Asp, Glu, Lys, Arg, or His: must be kept in a desiccator within a hermetically sealed vial since they absorb moisture from the atmosphere.